电离辐射通过再生生长因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

简短 ：反思远红外线否可通过裂解生长因子-β（TGF-β）-介导的黏膜-泌尿系统转换 (EMT)来促进细胞的肆虐迁移。采行据统计2Gy(60)Coγ线紫外线称做人类器官的6种细胞，记录与EMT相关的变化，这包括分别透过成像技术开发，抗原质；还有方法，免疫荧光技术开发，残余物测试和Transwell小室测试来推论并检测细胞组织形态，EMT标记，肆虐迁移技能等。采行抗原质联免疫吸附法检测这些细胞中会TGF-β抗原水平，透过特别抑制剂SB431542来评估TGF-β路径自营在远红外线EMT中会的作用。经过据统计为2Gy紫外线的细胞中会存在间叶细胞的表达，与假紫外线组相比其黏膜标记降低，间叶细胞标记增加，同时其肆虐转回技能增强，TGF-β抗原水平也提高。全面性发现由A549远红外线其会的EMT可通过对TGF-β路径抑制发生逆转。这些结果表明TGF-β介导的EMT在远红外线其会增强细胞肆虐转回技能中会起着关键性。

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